| Place | Name | | Affiliation | Time | | | Heat (Pl) |
|---|
| 1 | Molly Nanni | | Ss. Peter & Paul | 14.43 | Q | | 3 (1) |
| 2 | Mulani Johnson | | St Francis DeSale - Phila | 15.25 | Q | | 1 (1) |
| 3 | Ava Ceulers | | Pope John Paul II | 15.26 | q | | 1 (2) |
| 4 | Miyana King | | St Rose of Lima Philadelphia | 15.41 | Q | | 2 (1) |
| 5 | Julianne Snopkowski | | Sacred Heart Oxford | 15.48 | q | | 2 (2) |
| 6 | Alexis Walker | | St Martin dePorres | 15.65 | q | | 1 (3) |
| 7 | Katie McGovern | | St Genevieve | 15.67 | q | | 2 (3) |
| 8 | Molly O'Connor | | Assumption B V M-West Grove | 15.74 | q | | 2 (4) |
| 9 | Laila Lyons | | Our Mother of Consolation | 15.85 | | | 1 (4) |
| 10 | Milan Dukes | | St Helena-Incarnation | 16.01 | | | 3 (2) |
| 11 | Tajae Henricks | | St Rose of Lima Philadelphia | 16.18 | | | 3 (3) |
| 12 | Ava Coyle | | St Matthew | 16.40 (16.396) | | | 3 (4) |
| 13 | Sarah Hughes | | Christ the King | 16.40 (16.400) | | | 2 (5) |
| 14 | Olivia Lieu | | Immaculate Heart of Mary | 16.49 | | | 1 (5) |
| 15 | Emma DelVecchio | | Our Mother of Consolation | 16.56 | | | 1 (6) |
| 16 | Alexis Farrell | | St Martha | 16.86 | | | 3 (5) |
| 17 | Alyssa Mayberry | | Our Mother of Consolation | 16.88 (16.871) | | | 1 (7) |
| 18 | Madison Crozier | | St Anselm | 16.88 (16.876) | | | 3 (6) |
| 19 | Megan Comas | | St Christopher | 17.77 | | | 2 (6) |
| Kayla Backer | | St Patrick Malvern | DNS | | | 1 |
| Nahaiya Boyles | | St Martin dePorres | DNS | | | 2 |
| Emma Loscalzo | | Holy Martyrs | DNS | | | 2 |
| Lauren DiOttavio | | St Joseph-Downingtown | DNS | | | 3 |