| Place | Name | | Affiliation | Time | | | Heat (Pl) |
|---|
| 1 | Molly Nanni | | Ss. Peter & Paul | 29.71 | | | 3 (1) |
| 2 | Kalise Mack | | St Martin dePorres | 30.62 | | | 3 (2) |
| 3 | Julianne Snopkowski | | Sacred Heart Oxford | 32.51 | | | 2 (1) |
| 4 | Mulani Johnson | | St Francis DeSale - Phila | 32.71 | | | 3 (3) |
| 5 | Kristen Pineda | | Pope John Paul II | 32.93 | | | 3 (4) |
| 6 | Margaux Rawson | | Immaculate Conception | 33.16 | | | 2 (2) |
| 7 | Laila Lyons | | Our Mother of Consolation | 33.44 | | | 1 (1) |
| 8 | Allison Palmer | | St Christopher | 33.49 | | | 2 (3) |
| 9 | Alexis Walker | | St Martin dePorres | 33.71 | | | 2 (4) |
| 10 | Kate Eckerd | | Ss. Peter & Paul | 33.76 | | | 2 (5) |
| 11 | Miyana King | | St Rose of Lima Philadelphia | 34.43 | | | 3 (5) |
| 12 | Catherine Shuron | | St Genevieve | 34.64 (34.633) | | | 1 (2) |
| 13 | Olivia Lieu | | Immaculate Heart of Mary | 34.64 (34.636) | | | 3 (6) |
| 14 | Alyssa Mayberry | | Our Mother of Consolation | 34.99 | | | 1 (3) |
| 15 | Rebecca Dalton | | Our Lady of Calvary | 35.02 | | | 1 (4) |
| 16 | Alexa Stewart | | St Christopher | 35.12 | | | 1 (5) |
| 17 | Sage Green | | St Anselm | 35.20 | | | 1 (6) |
| 18 | Kristen Frain | | Maternity B V M | 35.29 | | | 2 (6) |
| 19 | Elizabeth Stampone | | St Jerome | 35.84 | | | 1 (7) |
| 20 | Emma DelVecchio | | Our Mother of Consolation | 36.02 | | | 2 (7) |
| Sarah Fedders | | Immaculate Conception | DNS | | | 1 |
| Lauren DiOttavio | | St Joseph-Downingtown | DNS | | | 2 |
| Nahaiya Boyles | | St Martin dePorres | DNS | | | 3 |
| Kayla Backer | | St Patrick Malvern | DNS | | | 3 |