| Place | Name | | Affiliation | Time | | | Heat (Pl) |
|---|
| 1 | Molly Nanni | | Ss. Peter & Paul | 14.11 | Q | | 2 (1) |
| 2 | Maura Dianno | | St Alphonsus | 14.13 | Q | | 3 (1) |
| 3 | Alexis Sapnas | | St Mary Magdalene | 14.25 | Q | | 1 (1) |
| 4 | Heather Lesinski | | St Eleanor | 14.56 | q | | 1 (2) |
| 5 | Udo Onyemachi | | Blessed Virgin Mary | 14.58 | q | | 3 (2) |
| 6 | Madison Angelo | | St Agnes / Sacred Heart | 14.64 | q | | 3 (3) |
| 7 | Aviana Principato | | St Andrew-Newtown | 14.74 | q | | 2 (2) |
| 8 | Lauren Fadgen | | Our Lady of Perpetual Help | 14.86 | q | | 1 (3) |
| 9 | Mulani Johnson | | St Francis DeSale - Phila | 15.08 | | | 1 (4) |
| 10 | Anna Patterson | | St Eleanor | 15.13 | | | 3 (4) |
| 11 | Natalie Coughlin | | Waldron Mercy | 15.18 | | | 3 (5) |
| 12 | Molly O'Connor | | Assumption B V M-West Grove | 15.21 | | | 1 (5) |
| 13 | Emma Devine | | St Michael the Archangel | 15.24 | | | 2 (3) |
| 14 | Ava Ceulers | | Pope John Paul II | 15.25 | | | 1 (6) |
| 15 | Caroline Colman | | St Andrew-Newtown | 15.26 | | | 2 (4) |
| 16 | Paige Hoeger | | St Catherine of Siena Horsham | 15.28 (15.274) | | | 2 (5) |
| 17 | Kiera Tavolaro | | St Alphonsus | 15.28 (15.276) | | | 1 (7) |
| 18 | Lauren Anderson | | Waldron Mercy | 15.36 | | | 3 (6) |
| 19 | Miyana King | | St Rose of Lima Philadelphia | 15.39 | | | 2 (6) |
| 20 | Claire Kutufaris | | St Francis of Assisi | 15.42 | | | 1 (8) |
| 21 | Katie McGovern | | St Genevieve | 15.49 | | | 2 (7) |
| 22 | Alexis Walker | | St Martin dePorres | 18.15 | | | 2 (8) |
| Julianne Snopkowski | | Sacred Heart Oxford | DNS | | | 3 |