| Place | Name | | Affiliation | Time | | | Heat (Pl) |
|---|
| 1 | Sara Biunno | | Our Lady of Fatima-Secane | 27.80 | | | 3 (1) |
| 2 | Liz Saldutti | | Corpus Christi | 28.52 | | | 3 (2) |
| 3 | Catie Catalino | | Our Lady of Mount Carmel | 28.57 | | | 3 (3) |
| 4 | Alyse Caffrey | | Holy Rosary Regional School | 28.61 | | | 3 (4) |
| 5 | Jayla Reeves | | St Martin dePorres | 29.02 | | | 3 (5) |
| 6 | Olivia Reinsel | | St Thomas the Apostle | 29.03 | | | 2 (1) |
| 7 | Kelsey Sebwe | | Maternity B V M | 29.18 | | | 2 (2) |
| 8 | Anne Jeanette Ardito | | Our Lady of Guadalupe | 29.29 | | | 2 (3) |
| 9 | Sabrina Rosado | | St Katherine of Siena-Phila | 29.47 | | | 1 (1) |
| 10 | Eva Webb | | Gesu | 29.79 | | | 2 (4) |
| 11 | Theresa Richardson | | Holy Family Regional-Levittown | 30.05 | | | 1 (2) |
| 12 | Alyssa Friedman | | St Charles Borromeo-Bensalem | 30.10 (30.091) | | | 1 (3) |
| 13 | Danielle Herbert | | St Martin of Tours - New Hope | 30.10 (30.099) | | | 2 (5) |
| 14 | Shannon Purfield | | St Joseph-Aston | 30.23 | | | 1 (4) |
| 15 | Amira Woodlin | | Gesu | 30.35 | | | 1 (5) |
| 16 | Keri Daly | | St Denis | 30.39 | | | 1 (6) |
| 17 | Madeline Kempski | | St Maximilian Kolbe | 30.52 | | | 2 (6) |
| 18 | Kemi Odeyemi | | St Dorothy | 30.66 | | | 1 (7) |
| 19 | Melina Trautman | | St Thomas the Apostle | 30.75 | | | 1 (8) |
| 20 | Kathleen Anderson | | Villa Maria Academy | 31.05 | | | 2 (7) |
| Avary Kalac | | St Ignatius | DNS | | | 2 |
| Elle Halfpenny | | Ss. Colman - John Neumann | DNS | | | 3 |
| Daja Hosendrof | | St Francis DeSale - Phila | DNS | | | 3 |
| Sophia Mahoney | | Immaculate Conception | DNS | | | 3 |